in e. coli, what is the function of dna polymerase iii? This is a topic that many people are looking for. star-trek-voyager.net is a channel providing useful information about learning, life, digital marketing and online courses …. it will help you have an overview and solid multi-faceted knowledge . Today, star-trek-voyager.net would like to introduce to you DNA polymerase 3. Following along are instructions in the video below:
Back friends in this video tutorial. Well be talking about the dna polymerase. 3.
3. Ok so lets talk about it dna polymerase. 3.
Is one of the dna polymerase that we find and polymerize enzyme. Is important for the dna. Replication process dna polymerase for the rna transcription.
We require rna polymerase. So polymerase enzyme is mainly the major function of polymerase enzyme is to polymerize nucleotide is to polymerize either dna or rna. If it polymerizes dna.
It could be called dna polymerase. Which polymerizes rna. It will be called rna.
Polymerase. Now. The polymer is that we see in case of the trip dna replication process in prokaryotes among them these two dna.
Polymerase c. And dna. Polymer is one our major type.
Which has a major function in gain replication in prokaryotes now among them also in prokaryotes the functionality of dna polymerase. 3. Is the most important part and dna.
Polymerase c. Is very much complex then dna polymer is one this is discovered letter in at the very beginning. We discovered the dna polymerase.
One discovered by arthur kornberg. Now after that many years later his son discovered this dna. Polymerase.
3. As you can see its very much complicated structure have different subunits. But there is some important thing i want to talk about here about the dna polymerase.
3. Is that this dna polymerase c have two identical almost kind of two identical hemisphere structures if you look at here you can find that things straight to different to separate zones. Why.
It has to difference are two separate arrangement as you can see alpha epsilon and theta and same thing. All fictional theta and they are linked those two units. They are linked with structure called tau tau region or tau subunit of the protein will fold.
Those two hemispheres together and these two different positions are required for two different strands for the dna. Because the dna synthesis in eukaryotes as well as in prokaryotes occur simultaneously in both of the strand. One from 5 prime to 3 prime another from the opposite.
But also it forms a loop to counter attack that and synthesized sim same time simultaneously both the strands. Though one strand. The synthesis continuous and called the leading strand.
Other strand synthesis discontinuous and called lagging strength. So. The structure is very important and the town subunit just remember.
This is holding everything. Together and another important structure. Here this this region of chi.
Sai delta sigma and all this delta gamma. So this is the structure. Which is called as a dna loader.
So once the dna is dissociated hemic is actually placed somewhere here. Which dissociates the both of the strands of the dna. So until helices a dnas double stranded after that the dna becomes single stranded.
So that is a function of helices. So once eliquis is done then the single stranded dna is loaded to one of the arms of this dna. Polymerase.
3. By this particular section. This is called the hollow hands and the hollow enzyme.
There so. If you look at here. This is a core enzyme.
Actually and halogen will be complete. If i add a draw structure here. Which is called as the beta clamp this is called beta clamp b.
Kirkland has a specific protein sequence structure just looking like a clamp which will hold that is like a clip hold on to cinder strand of the dna. And due to the presence of this beta clamps and some proteins here theta epsilon. They call as a clamp loader protein with the help of this proteins.
They load this clamp. The top of the single stranded dna and can easily start. Doing a start.
Adding. Nucleotide sequences. And continue this process of dna replication.
The dna polymerization for a long time. This is the important property of dna polymerase. 3.
And this property is the higher processivity dna polymerase ii have much higher processing facility than the dna polymerase 1 due to the presence of beta clamp and clamp. Loaded proteins and these are the police presence of this particular structure so except for that this is the polymerization part so the functionality of dna polymerase. 3.
Here is the polymerization and we have talked about it how helices helps and how beta camp help this process. If you want to know more details about this process. I will recommend you to go back.
And you will find video on dna replication in my youtube channel watch that video. And you will understand the process. So.
If you have the polymerization process in this polymerization process. This polymerization direction here is 5 prime to 3 prime. This is the only disadvantage for this dna.
Polymerase. 3. The polymerization is only possible from 5 prime to 3 prime as well as they have another disadvantages they cannot this polymerase.
3. Cannot initiate base pairing it cannot initiate the nucleotide addition until and unless they find a free 3 prime hydroxyl group. So for the continuation of the polymer as i said.
This is the template strand for the polymerization process by looking at the single stranded dna. The polymerase cannot initiate. Lets say everything is there dna.
Polymerase is also there. But the polymerase cannot initiate a material unless it finds. What is 4 of primer.
The primer have a c prime. Hydroxyl region and then with the help of this c. Prime hydroxyl.
This dna polymerase can extend the chain. But cannot initiate on the other hand rna. Polymerase can initiate from scratch.
So. Do you know how polymerase is rna. Polymerase.
This dna. Polymerase is not de novo. They require the stretch of nucleotide sequence.
As primer. Now. This is one disadvantage about other things other functionality here is the exonuclease functionality exonuclease means if this is a dna or say rna whatever in this case of dna it so nucleus.
So this is the dna it can cleave from outside. I mean from timing it can cleave that dna thats called the exonuclease activity can degrade the nucleotide sequence. There.
So why. It is important. It has a constructive feature polymerization destructive.
Feature exonuclease activity. Why it is required because during the replication process. If it is erroneously add a mismatch inordinately add a wrong nucleotide let us say what we know the process actually follows.
The watson crick base pairing right so if we have like this. And it is placing all the required so all the required nucleotides. Its a tcg g.
Instead of a it provides a seizure. So let us say it made a mistake here so it make any mistake during this process of polymerization. This polymer is enzyme can sense that how can this sense.
The sensing is possible because every time it puts a wrong nucleotide. The the processivity goes slow and slow so due to this process of how many times. Its take to to place this nucleotide and form hydrogen bonds.
It counts. The whether it is any problem or not it finds the problem then this polymerase shifts its structure. Slightly.
So that there are different domains in this polymer. Is actually catalytic domain. Is the polymerization domain.
Exonuclease domain. And also finger domain. Which is placing the dna.
Properly right so this is much more complicated dna polymerase. 3. But if you look at the structure of dna.
Polymerase. 1. You will find the structure resembles just like a hand one finger.
I mean thumb fingers and the form fermi is usually the catalytic domain. So it can sense the wrong nucleotide. It can come back and then the exonuclease activity takes place and they will cut this wrong nucleotide away and then they put the right nucleotide in the position that is why the exonuclease activity is required right so that is how the dna polymerase.
3. Is there and this exonuclease activity direction for the dna polymerase is however opposite. 3 prime to 5 prime that is logical because if you have the polymerization activity from 5 to 3.
And also exonuclease from pi plus. It can have some problem so if it is it is adding something in the forward. 5 to 3 prime.
It has some problem go back and cut the first chip time nucleotide out so that is completely logical to have right. But remember the dna polymer is one besides having 3 prime to 5 prime exonuclease activity. Polymer is one also have 3 to 5 prime exonuclease activity.
Polymerase ii. Only have 3 to 5 prime exonuclease. But polymer is one half 5 prime to 3 prime exonuclease along with this 3 to 5 prime.
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